Fuyuan Huoxue Decoction Promotes Skin Flap Survival Via the P38MAPK/IKK/NF-κB Pathway in Rats

Objective Fuyuan Huoxue decoction (FHD) is a traditional Chinese medicine that can promote blood circulation, inhibit inflammatory reactions and effectively relieve pain. This study investigated the effect of FHD on rat skin flap survival p38 MAPK/IKK/NF-κB pathway. Methods The pathological condition tissue was examined by H&E staining. expression levels CD34 VEGF in tissues were immunohistochemistry. TNF-α, IL-6, CRP, HO-1, superoxide dismutase (SOD) activity, MDA content detected ELISA. protein Bax, Bcl-2, cleaved-caspase 3, p-IKKαβ, p-p38, p-p65, α-SMA, CK14, CK15, SOX2 western blotting. Results upregulated CD34, VEGF, downregulated P-P38, P-P65, increased activity SOD, inhibited level MDA. In addition, promoted proliferation differentiation flap. Conclusions improved model regulating p38MAPK/IKK/NF-κB signaling pathway, mechanism may be related to promotion proliferation, differentiation, revascularization inhibition inflammation oxidative stress.